Ĭozza G, Pinna LA (2016) Casein kinases as potential therapeutic targets. Ruzzene M, Pinna LA (2010) Addiction to protein kinase CK2: a common denominator of diverse cancer cells? Biochim Biophys Acta 3:499–504. Mol Cell Biochem 191(1–2):65–74Ĭhannavajhala P, Seldin DC (2002) Functional interaction of protein kinase CK2 and c-Myc in lymphomagenesis. Xu X, Landesman-Bollag E, Channavajhala PL, Seldin DC (1999) Murine protein kinase CK2: gene and oncogene. Ortega CE, Seidner Y, Dominguez I (2014) Mining CK2 in cancer. Litchfield DW (2003) Protein kinase CK2: structure, regulation and role in cellular decisions of life and death. Pinna LA (2003) The raison d’etre of constitutively active protein kinases: the lesson of CK2. St-Denis NA, Litchfield DW (2009) Protein kinase CK2 in health and disease: from birth to death: the role of protein kinase CK2 in the regulation of cell proliferation and survival. Pinna LA (2002) Protein kinase CK2: a challenge to canons. They also show that the lack of CK2 promotes phosphoproteomics perturbations attributable to kinases other than CK2.Īllende CC, Allende JE (1998) Promiscuous subunit interactions: a possible mechanism for the regulation of protein kinase CK2. Collectively taken our data allow to conclude that the phosphoproteome generated by CK2 is not as ample and rigidly pre-determined as it was believed before. We also find that phosphosites notoriously generated by CK2 are not fully abrogated in CK2α/α′ (−/−) cells, while some phosphosites unrelated to CK2 are significantly altered.
A parallel study with C2C12 cells deprived of the regulatory β subunit discloses a role of this subunit in determining CK2 targeting. A comparative SILAC phosphoproteomics analysis reveals that, although about 30% of the quantified phosphosites do conform to the CK2 consensus, only one-third of these are substantially reduced in the CK2α/α′ (−/−) cells, consistent with their generation by CK2. To gain information about this issue, advantage has been taken of C2C12 cells entirely deprived of both CK2 catalytic subunits by the CRISPR/Cas9 methodology. The CK2 consensus sequence, S/T-x-x-D/E/pS/pT is present in numerous phosphosites, but it is not clear how many of these are really generated by CK2. CK2 denotes a ubiquitous and pleiotropic protein kinase whose holoenzyme is composed of two catalytic (α and/or α′) and two regulatory β subunits.
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